Characterization and Evaluation of Antimicrobial Activity of Sorbitan Isolated from the Leaves of Securidaca longipedunculata (Fresen) (retracted)

Abstract

Securidaca longipedunculata is a shrub of the family Polygalaceae. It is a
potent medicinal plant used in management of microbial infection others.
The aim of this study is to characterize, and evaluate the antimicrobial
activity of sorbitan isolated from the leaves of Securidaca Longipedunculata
(Fresen). The methanol extract of S. longipedunculata was subjected to
chromatographic purification, which led to isolation of compound identified
as sorbitan by analysis of the 1D and 2D-NMR spectral data. The
antimicrobial screening against bacterial pathogens (Methicillin-resistant
staphylococcus aureus (MRSA), Vancomycin-resistant enterococci (VRE) S.
aureus, S. feacalis, E. coli, S. typhimurium, P. fluorescens, K. pneumoniea)
and fungal pathogens (C. albicans, C. krusei, A. niger, A. fumigates, and
Microsporumcanis) was performed using agar well diffusion and broth
dilution method. In-vitro inhibition of these pathogenic microorganisms
produced inhibition zone ranging from 25 - 32 mm for sorbitan; the standard
drugs sparfloxacin and ciprofloxacin (500µg/mL) had zone of inhibition
ranging from 28 - 40 mm. While the antifungal drugs fluconazole and Fulcin
exhibited zone of inhibition ranging from 27 mm to 34 mm. Minimum
inhibition concentration (MIC) values of sorbitan ranged from 25 - 12.5
mg/mL. The minimum bactericidal concentration (MBC)/minimum fungicidal
concentration (MFC) of sorbitan was observed to be 50 - 25 mg/mL.
Sorbitan was isolated and characterized from S. longipedunculata leaves
extract and it demonstrated good antimicrobial activity which validates the
used of the plant leaves in the treatment of microbial infections in traditional
medicine